Anti-Asparaginase antibody (Biotin) (ab34616)

Overview

  • Product nameAnti-Asparaginase antibody (Biotin)
    See all Asparaginase primary antibodies
  • Description
    Rabbit polyclonal to Asparaginase (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Immunomicroscopy, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Asparaginase (Escherichia coli )

Properties

Applications

Our Abpromise guarantee covers the use of ab34616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 45957
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceAsparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
  • Cellular localizationCytoplasmic, Secreted and Periplasm.
  • Database links
    • Alternative names
      • AnsA antibody
      • AnsB antibody
      • Colaspase antibody
      • Cytoplasmic asparaginase I antibody
      • L ASNase I antibody
      • L ASNase II antibody
      • L asparaginase 1 antibody
      • L asparaginase 2 antibody
      • L asparaginase I antibody
      • L asparaginase II antibody
      • L asparaginase II precursor antibody
      • L asparagine amidohydrolase I antibody
      • L asparagine amidohydrolase II antibody
      see all

    Anti-Asparaginase antibody (Biotin) images

    • ab34616 staining Asparaginase in Mice treated with E.coli Asparaginase or mock treated mice were sacrificed 16 hours after injection. Tissue was stained for Asparaginase uptake by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2% Normal Goat serum + 2% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 1 hours at 20°C.

      See Abreview

    References for Anti-Asparaginase antibody (Biotin) (ab34616)

    ab34616 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step (agent) for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate, pH6
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2014

    Thank you for your enquiry. I have contacted the originator of this antibody and unfortunately we do not know which asparaginase this antibody is specific to; we only know that it reacts against asparaginase in E. coli. I am sorry that we do not have m...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"