Anti-Asparagine synthetase antibody (ab111873)

Overview

  • Product name
    Anti-Asparagine synthetase antibody
    See all Asparagine synthetase primary antibodies
  • Description
    Rabbit polyclonal to Asparagine synthetase
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 358-407 (SVVIFSGEGS DELTQGYIYF HKAPSPEKAE EESERLLREL YLFDVLRADR) of Human Asparagine synthetase (NP_001664).

  • Positive control
    • Human Fetal Lung Lysate. IF/ICC: U-87 MG cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab111873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Pathway
    Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
  • Sequence similarities
    Contains 1 asparagine synthetase domain.
    Contains 1 glutamine amidotransferase type-2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • asnS antibody
    • ASNS_HUMAN antibody
    • ASNSD antibody
    • Asparagine synthetase [glutamine-hydrolyzing] antibody
    • Asparagine synthetase antibody
    • Cell cycle control protein TS11 antibody
    • Glutamine dependent asparagine synthetase 3 antibody
    • Glutamine dependent asparagine synthetase antibody
    • Glutamine hydrolyzing antibody
    • Glutamine-dependent asparagine synthetase antibody
    • OTTHUMP00000024510 antibody
    • OTTHUMP00000204938 antibody
    • OTTHUMP00000204939 antibody
    • OTTHUMP00000204940 antibody
    • OTTHUMP00000204941 antibody
    • OTTHUMP00000204942 antibody
    • TS11 antibody
    • TS11 cell cycle control protein antibody
    see all

Images

  • ICC/IF image of ab11873 stained U-87 MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111873, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Asparagine synthetase antibody (ab111873) at 1 µg/ml + Human Fetal Lung Lysate at 10 µg

    Predicted band size : 64 kDa

References

ab111873 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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