Anti-Asparagine synthetase antibody (ab126254)
Key features and details
- Rabbit polyclonal to Asparagine synthetase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Asparagine synthetase antibody
See all Asparagine synthetase primary antibodies -
Description
Rabbit polyclonal to Asparagine synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to region within amino acids 1 -227 of the Human Asparagine synthetase protein (P08243).
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Positive control
- K562 cell lysates and Human Cal27 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 64 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Pathway
Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1. -
Sequence similarities
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain. - Information by UniProt
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Database links
- Entrez Gene: 440 Human
- Omim: 108370 Human
- SwissProt: P08243 Human
- Unigene: 489207 Human
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Alternative names
- asnS antibody
- ASNS_HUMAN antibody
- ASNSD antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab126254 has been referenced in 3 publications.
- Xiong J et al. SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential. EBioMedicine 72:103614 (2021). PubMed: 34628354
- Ruiz-Rodado V et al. Metabolic plasticity of IDH1-mutant glioma cell lines is responsible for low sensitivity to glutaminase inhibition. Cancer Metab 8:23 (2020). PubMed: 33101674
- Du F et al. SOX12 promotes colorectal cancer cell proliferation and metastasis by regulating asparagine synthesis. Cell Death Dis 10:239 (2019). PubMed: 30858360