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Rabbit polyclonal to Asparagine synthetase
Recombinant fragment, corresponding to region within amino acids 1 -227 of the Human Asparagine synthetase protein (P08243).
- K562 cell lysates and Human Cal27 xenograft.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 64 kDa.
||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain.
Information by UniProt
- asnS antibody
- ASNS_HUMAN antibody
- ASNSD antibody
Western blot - Anti-Asparagine synthetase antibody (ab126254)
Anti-Asparagine synthetase antibody (ab126254) at 1/1000 dilution + K562 whole cell lysate at 30 µg
Predicted band size: 64 kDa
Gel concentration: 7.5%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase antibody (ab126254)
ab126254, at 1/500, staining Asparagine synthetase in Human Cal27 xenograftby immunohistochemistry [Paraffin Embedded Tissues (IHC-P).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"