Recombinant
RabMAb

Anti-Asparagine synthetase antibody [EP282Y] (ab40850)

Overview

  • Product name
    Anti-Asparagine synthetase antibody [EP282Y]
    See all Asparagine synthetase primary antibodies
  • Description
    Rabbit monoclonal [EP282Y] to Asparagine synthetase
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human Asparagine synthetase.

  • Positive control
    • K562 cell lysate, human breast carcinoma tissue and human muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available: 
    Anti-Asparagine synthetase antibody (Alexa Fluor® 647) [EP282Y] (ab207508)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab40850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Pathway
      Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
    • Sequence similarities
      Contains 1 asparagine synthetase domain.
      Contains 1 glutamine amidotransferase type-2 domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • asnS antibody
      • ASNS_HUMAN antibody
      • ASNSD antibody
      • Asparagine synthetase [glutamine-hydrolyzing] antibody
      • Asparagine synthetase antibody
      • Cell cycle control protein TS11 antibody
      • Glutamine dependent asparagine synthetase 3 antibody
      • Glutamine dependent asparagine synthetase antibody
      • Glutamine hydrolyzing antibody
      • Glutamine-dependent asparagine synthetase antibody
      • OTTHUMP00000024510 antibody
      • OTTHUMP00000204938 antibody
      • OTTHUMP00000204939 antibody
      • OTTHUMP00000204940 antibody
      • OTTHUMP00000204941 antibody
      • OTTHUMP00000204942 antibody
      • TS11 antibody
      • TS11 cell cycle control protein antibody
      see all

    Images

    • Anti-Asparagine synthetase antibody [EP282Y] (ab40850) at 1/2000 dilution + K562 cell lysate at 10 µg

      Predicted band size : 61 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab40850 at a 1:50 dilution staining Asparagine synthetase in human breast carcinoma tissue.
    • ab40850 at a 1:50 dilution staining Asparagine synthetase in human muscle tissue.
    • Immunohistochemical analysis of Human stomach tissue, staining Asparagine synthetase with ab40850. Tissue was blocked with goat serum before incubating with primary antibody (1/50) overnight at room temperature. Staining was detected using DAB.

    References

    This product has been referenced in:
    • Huang ML  et al. Molecular and functional alterations in a mouse cardiac model of Friedreich ataxia: activation of the integrated stress response, eIF2a phosphorylation, and the induction of downstream targets. Am J Pathol 183:745-57 (2013). WB ; Mouse . Read more (PubMed: 23886890) »
    • Scotti C  et al. Cell-cycle inhibition by Helicobacter pylori L-asparaginase. PLoS One 5:e13892 (2010). WB, IHC-P ; Human . Read more (PubMed: 21085483) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HL60)
    Loading amount
    50 µg
    Specification
    HL60
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 09 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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