Overview

  • Product name
    Aspartate Aminotransferase Activity Assay Kit
    See all Aspartate Aminotransferase kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Enzyme activity
  • Sensitivity
    10 mU/well
  • Assay duration
    Multiple steps standard assay
  • Product overview

    In Abcam's Aspartate Aminotransferase Activity Assay Kit an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (?max = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of AST with a detection limit of 10 mU per well.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab105135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).

  • AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.

  • AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.

  • AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).

  • Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.

  • Example of Standard Curve and Positive control detection using ab105135.

Protocols

References

This product has been referenced in:
  • Smedlund K  et al. Early steatohepatitis in hyperlipidemic mice with endothelial-specific gain of TRPC3 function precedes changes in aortic atherosclerosis. Physiol Genomics 48:644-9 (2016). Read more (PubMed: 27449657) »

See 1 Publication for this product

Customer reviews and Q&As



It is recommended to measure standard curve at both times 10 mins and 60 mins. Then use the difference in Abs at 60min - Abs at 10min to plot the Std curve (OD on Y axis versus Glutamate nmol on the X axis).

Please let me know if yo...

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The identity of all the kit components is proprietary information. The mechanism of the reaction is exactly as mentioned on the datasheet. The substrate is a sequence the AST and the other enzymes in the provided mix will act upon. The probe will inter...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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