Overview

  • Product nameAspartate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 2 µM
  • Range
    2 µM - 200 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle, participates in gluconeogenesis and transports reducing equivalents between the cytosol and the mitochondria via the malate-aspartate shuttle. Aspartate also stimulates NMDA receptors (not as strongly as glutamate) and hence serves as an excitatory neurotransmitter in the brain and is an excitotoxin.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab102512 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent dilution.

    Aspartate Assay Kit images

    • Colorimetric Standard Curve Performed as Described in the Protocol
    • Fluorometric Standard Curve Performed as Described in the Protocol

    Protocols

    References for Aspartate Assay Kit (ab102512)

    ab102512 has not yet been referenced specifically in any publications.

    Product Wall



    Start with ˜2X10^6 cells, suspend the cell pellet 500 μl of the aspartate assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the microscope. Spin...

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    Reconstituted Serum CleanUp Mix, Aspartate Enzyme Mix and Conversion Mix can be kept for up to a week at 4C after reconstitution. At -20C these components will be stable for 2 months.

    The stock...

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    I can confirm that all three kits have not been especially tested with frozen serum samples.

    I believe that there should be no problem for the Urea Assay Kit and Aspartate Assay Kit.

    For the Ala...

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    This kit is different than aspartate transminase activity kit. it will not detect the aspartate transminase enzyme.

    ab102512 based on the fact that the enzyme mix converts aspartate to pyruvate with the...

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    We have ab105134 Alanine Transaminase Activity Assay Kitwhich should fit your experiment, as it can be used in mouse:

    http://www.abcam.com/index.html?datasheet=105134 (or use the following: http://www.a...

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    Thank you for your inquiry. I can confirm that all these kits are suitable for mouse samples: ab83362 and ab102512: These kits can be used with a variety of samples such as serum, plasma, and urine and should not be species dependent. As long as there ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"