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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12797
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab172475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).
ab172475 has not yet been referenced specifically in any publications.