Overview

  • Product name
  • Description
    Rabbit polyclonal to ASPP1
  • Specificity
    Minimal reactivity occurs against ASPP2.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of ASPP1 (Human)

  • Positive control
    • MCF7 cells extracts

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Sterile filtered.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Detects a band of approximately 120 kDa (predicted molecular weight: 121 kDa).
ELISA 1/2500 - 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
  • Tissue specificity
    Reduced expression in breast carcinomas expressing a wild-type TP53 protein.
  • Sequence similarities
    Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • Domain
    The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-stimulating of p53 protein 1 antibody
    • ASPP1_HUMAN antibody
    • p53BP2 like antibody
    • p85 antibody
    • Ppp1r13b antibody
    • Protein phosphatase 1 regulatory subunit 13B antibody
    see all

Images

  • All lanes : Anti-ASPP1 antibody (ab51831) at 1/1000 dilution

    Lane 1 : Non-transfected control
    Lane 2 : MCF7 cells over expressing ASPP1
    Lane 3 : MCF7 cells overexpressing ASPP2


    Predicted band size : 121 kDa
    Observed band size : 50,120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 121 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab51831 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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