The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/500 - 1/3000. Detects a band of approximately 120 kDa (predicted molecular weight: 121 kDa).
1/2500 - 1/10000.
Use at an assay dependent concentration.
Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
Reduced expression in breast carcinomas expressing a wild-type TP53 protein.
Belongs to the ASPP family. Contains 2 ANK repeats. Contains 1 SH3 domain.
The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.