Anti-ASPP1 antibody (ab71163)
Key features and details
- Rabbit polyclonal to ASPP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASPP1 antibody
See all ASPP1 primary antibodies -
Description
Rabbit polyclonal to ASPP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
A synthetic peptide corresponding to a region between residue 50 and 100 of human ASPP1 (NP_056131.1)
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Positive control
- Whole cell lysate from 293T cells and Hela cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. -
Tissue specificity
Reduced expression in breast carcinomas expressing a wild-type TP53 protein. -
Sequence similarities
Belongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain. -
Domain
The ankyrin repeats and the SH3 domain are required for specific interactions with TP53. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear. - Information by UniProt
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Database links
- Entrez Gene: 23368 Human
- Omim: 606455 Human
- SwissProt: Q96KQ4 Human
- Unigene: 709297 Human
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Alternative names
- Apoptosis-stimulating of p53 protein 1 antibody
- ASPP1_HUMAN antibody
- p53BP2 like antibody
see all
Images
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All lanes : Anti-ASPP1 antibody (ab71163) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 5 µg
Lane 4 : Whole cell lysate from Hela cells at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation/ Western Blot of ASPP1.
Lane 1: ab71163 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
All lanes: Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab71163 has been referenced in 2 publications.
- Chen D et al. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3. Cell Death Differ 20:931-940 (2013). PubMed: 23558950
- Vigneron AM & Vousden KH An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular senescence. EMBO J : (2011). WB ; Human . PubMed: 22068052