• Product name
  • Description
    Rabbit polyclonal to ASPP1
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residue 50 and 100 of human ASPP1 (NP_056131.1)

  • Positive control
    • Whole cell lysate from 293T cells and Hela cells.



Our Abpromise guarantee covers the use of ab71163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function
    Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
  • Tissue specificity
    Reduced expression in breast carcinomas expressing a wild-type TP53 protein.
  • Sequence similarities
    Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • Domain
    The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-stimulating of p53 protein 1 antibody
    • ASPP1_HUMAN antibody
    • p53BP2 like antibody
    • p85 antibody
    • Ppp1r13b antibody
    • Protein phosphatase 1 regulatory subunit 13B antibody
    see all

Anti-ASPP1 antibody images

  • All lanes : Anti-ASPP1 antibody (ab71163) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 5 µg
    Lane 4 : Whole cell lysate from Hela cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 121 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)

    Exposure time : 3 minutes
  • Immunoprecipitation/ Western Blot of ASPP1.
    Lane 1: ab71163 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    All lanes: Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • ICC/IF image of ab71163 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71163, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ASPP1 antibody (ab71163)

This product has been referenced in:
  • Chen D  et al. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3. Cell Death Differ 20:931-940 (2013). Read more (PubMed: 23558950) »
  • Vigneron AM & Vousden KH An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular senescence. EMBO J : (2011). WB ; Human . Read more (PubMed: 22068052) »

See all 2 Publications for this product

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