Overview

  • Product nameAnti-ASPP1 antibody
    See all ASPP1 primary antibodies
  • Description
    Rabbit polyclonal to ASPP1
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residue 50 and 100 of human ASPP1 (NP_056131.1)

  • Positive control
    • Whole cell lysate from 293T cells and Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionRegulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
  • Tissue specificityReduced expression in breast carcinomas expressing a wild-type TP53 protein.
  • Sequence similaritiesBelongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • DomainThe ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-stimulating of p53 protein 1 antibody
    • ASPP1_HUMAN antibody
    • p53BP2 like antibody
    • p85 antibody
    • Ppp1r13b antibody
    • Protein phosphatase 1 regulatory subunit 13B antibody
    see all

Anti-ASPP1 antibody images

  • All lanes : Anti-ASPP1 antibody (ab71163) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 5 µg
    Lane 4 : Whole cell lysate from Hela cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 121 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Immunoprecipitation/ Western Blot of ASPP1.
    Lane 1: ab71163 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    All lanes: Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • ICC/IF image of ab71163 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71163, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ASPP1 antibody (ab71163)

This product has been referenced in:
  • Chen D  et al. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3. Cell Death Differ 20:931-940 (2013). Read more (PubMed: 23558950) »
  • Vigneron AM & Vousden KH An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular senescence. EMBO J : (2011). WB ; Human . Read more (PubMed: 22068052) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab71163.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"