Overview

  • Product name
  • Description
    Rabbit polyclonal to ASPP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residue 475 and 525 of human ASPP1 (NP_056131.1)

  • Positive control
    • Whole cell lysate from 293T cells and Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
  • Tissue specificity
    Reduced expression in breast carcinomas expressing a wild-type TP53 protein.
  • Sequence similarities
    Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • Domain
    The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-stimulating of p53 protein 1 antibody
    • ASPP1_HUMAN antibody
    • p53BP2 like antibody
    • p85 antibody
    • Ppp1r13b antibody
    • Protein phosphatase 1 regulatory subunit 13B antibody
    see all

Images

  • All lanes : Anti-ASPP1 antibody (ab71164) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from 293T cells at 50 µg
    Lane 2 : Whole cell lysate from 293T cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 5 µg
    Lane 4 : Whole cell lysate from Hela cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 121 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Immunoprecipitation/ Western Blot of ASPP1.
    Lane 1: ab71164 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    All lanes: Whole cell lysate from 293T cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab71164 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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