The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRegulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
Tissue specificityReduced expression in breast carcinomas expressing a wild-type TP53 protein.
Sequence similaritiesBelongs to the ASPP family. Contains 2 ANK repeats. Contains 1 SH3 domain.
DomainThe ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.