Overview

  • Product name
  • Description
    Rabbit polyclonal to ASPP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 899-948 (EFDLVQRIIY EVEDPSKPND EGITPLHNAV CAGHHHIVKF LLDFGVNVNA) of Human ASPP1, NP_056131.

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
  • Tissue specificity
    Reduced expression in breast carcinomas expressing a wild-type TP53 protein.
  • Sequence similarities
    Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • Domain
    The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis-stimulating of p53 protein 1 antibody
    • ASPP1_HUMAN antibody
    • p53BP2 like antibody
    • p85 antibody
    • Ppp1r13b antibody
    • Protein phosphatase 1 regulatory subunit 13B antibody
    see all

Images

  • Anti-ASPP1 antibody (ab85535) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 121 kDa
    Observed band size : 121 kDa
    Additional bands at : 60 kDa,80 kDa. We are unsure as to the identity of these extra bands.

References

ab85535 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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