Validated using a knockout cell line

Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900)


  • Product name
    Anti-ASS1 antibody [EPR12399(B)] - C-terminal
    See all ASS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12399(B)] to ASS1 - C-terminal
  • Host species
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ASS1 aa 350 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P00966

  • Positive control
    • HeLa, Jurkat and fetal liver cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab170900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Pathway
      Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
      Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
    • Involvement in disease
      Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
    • Sequence similarities
      Belongs to the argininosuccinate synthase family. Type 1 subfamily.
    • Information by UniProt
    • Database links
    • Alternative names
      • Argininosuccinate synthase 1 antibody
      • Argininosuccinate synthase antibody
      • Argininosuccinate synthetase 1 antibody
      • ASS antibody
      • Ass-1 antibody
      • ass1 antibody
      • ASSA antibody
      • ASSY_HUMAN antibody
      • Citrulline aspartate ligase antibody
      • Citrulline--aspartate ligase antibody
      • CTLN1 antibody
      see all


    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab170900 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab170900 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1X PBS (negative control) using ab170900 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • All lanes : Anti-ASS1 antibody [EPR12399(B)] - C-terminal (ab170900) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa


    ab170900 has not yet been referenced specifically in any publications.

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