Anti-ASXL1 antibody (ab55825)

Overview

  • Product name
  • Description
    Rabbit polyclonal to ASXL1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to C-terminal residues of human ASXL1.

Properties

Applications

Our Abpromise guarantee covers the use of ab55825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 165 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Probable Polycomb group (PcG) protein. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1).
  • Tissue specificity
    Widely expressed at low level. Expressed in heart, brain, skeletal muscle, placenta, pancreas, spleen, prostate, small intestine, colon, peripheral blood, leukocytes, bone marrow and fetal liver. Highly expressed in testes.
  • Sequence similarities
    Belongs to the Asx family.
    Contains 1 PHD-type zinc finger.
  • Domain
    Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which may be required for an association with nuclear receptors.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Additional sex combs like 1 (Drosophila) antibody
    • Additional sex combs like protein 1 antibody
    • Additional sex combs-like protein 1 antibody
    • ASXL1 antibody
    • ASXL1_HUMAN antibody
    • KIAA0978 antibody
    • Putative Polycomb group protein ASXL1 antibody
    see all

References for Anti-ASXL1 antibody (ab55825)

ab55825 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Leukemia Cell Lines)
Loading amount
60 µg
Specification
Leukemia Cell Lines
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dipmoy Nath

Verified customer

Submitted Sep 19 2012

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I am very pleased to hear you would like to accept our offer and test ab55825 in mouse and/or human. This code will give you: 1 free PRIMARY ANTIBODY befor...

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