Overview

  • Product nameAnti-ASXL2 antibody
    See all ASXL2 primary antibodies
  • Description
    Mouse polyclonal to ASXL2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human ASXL2 protein (AAH42999, 1 a.a. ~ 918 a.a)

  • Positive control
    • ASXL2 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: Isoform 2, 98 kDa; Isoform 1, 154 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPutative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility.
    • Involvement in diseaseNote=A chromosomal aberration involving ASXL2 is a cause of therapy-related myelodysplastic syndrome. Translocation t(2;8)(p23;p11.2) with MYST3 generates a MYST3-ASXL2 fusion protein.
    • Sequence similaritiesBelongs to the Asx family.
      Contains 1 PHD-type zinc finger.
    • DomainContains two Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which may be required for an association with nuclear receptors.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • additional sex combs like 2 (Drosophila) antibody
      • Additional sex combs like 2 antibody
      • Additional sex combs like protein 2 antibody
      • Additional sex combs-like protein 2 antibody
      • ASXH2 antibody
      • ASXL 2 antibody
      • ASXL2 antibody
      • ASXL2_HUMAN antibody
      • DKFZp686C1968 antibody
      • FLJ10898 antibody
      • KIAA1685 antibody
      • Polycomb group protein ASXH2 antibody
      • Putative Polycomb group protein ASXL2 antibody
      see all

    Anti-ASXL2 antibody images

    • All lanes : Anti-ASXL2 antibody (ab69420) at 1/500 dilution

      Lane 1 : ASXL2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 98 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-ASXL2 antibody (ab69420)

    ab69420 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab69420.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"