Anti-ATAD1 antibody [N125/10] (ab94583)

Overview

  • Product name
    Anti-ATAD1 antibody [N125/10]
    See all ATAD1 primary antibodies
  • Description
    Mouse monoclonal [N125/10] to ATAD1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-361 (full length) of Mouse ATAD1

  • Positive control
    • Rat brain lysate
  • General notes

    The clone number has been updated from S125-10 to N125/10, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N125/10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • Application notes
    ICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 40 kDa.
    Note: Using the lysate without boiling may provide improved results.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.
    • Sequence similarities
      Belongs to the AAA ATPase family.
    • Cellular localization
      Peroxisome. Cell junction, synapse, postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4921525H23Rik antibody
      • AAA+ ATPase Thorase antibody
      • Ab2-088 antibody
      • AFDC1 antibody
      • Atad1 antibody
      • ATAD1_HUMAN antibody
      • ATPase family AAA domain containing protein 1 antibody
      • ATPase family AAA domain-containing protein 1 antibody
      • ATPase family, AAA domain containing 1 antibody
      • ATPase Thorase antibody
      • AW107648 antibody
      • FLJ14600 antibody
      • FNP001 antibody
      • Thorase antibody
      see all

    Images

    • Western blot detection of Atad1 in Rat brain membrane tissues using ab94583 at 1:1000 dilution.

    References

    ab94583 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Loading amount
    25 µg
    Specification
    293T
    Treatment
    siRNA knockdown
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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