Anti-ATAD1 antibody [S125-10] (ab94583)

Overview

  • Product nameAnti-ATAD1 antibody [S125-10]
  • Description
    Mouse monoclonal [S125-10] to ATAD1
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-361 (full length) of Mouse ATAD1

  • Positive control
    • Rat brain lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberS125-10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • Application notesICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 40 kDa.
    Note: Using the lysate without boiling may provide improved results.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.
    • Sequence similaritiesBelongs to the AAA ATPase family.
    • Cellular localizationPeroxisome. Cell junction, synapse, postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4921525H23Rik antibody
      • AAA+ ATPase Thorase antibody
      • Ab2-088 antibody
      • AFDC1 antibody
      • Atad1 antibody
      • ATAD1_HUMAN antibody
      • ATPase family AAA domain containing protein 1 antibody
      • ATPase family AAA domain-containing protein 1 antibody
      • ATPase family, AAA domain containing 1 antibody
      • ATPase Thorase antibody
      • AW107648 antibody
      • FLJ14600 antibody
      • FNP001 antibody
      • Thorase antibody
      see all

    Anti-ATAD1 antibody [S125-10] images



    • Predicted band size : 40 kDa
      Western blot detection of Atad1 in Rat brain membrane tissues using ab94583 at 1:1000 dilution.

    References for Anti-ATAD1 antibody [S125-10] (ab94583)

    ab94583 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T)
    Loading amount 25 µg
    Specification 293T
    Treatment siRNA knockdown
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Oct 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"