The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22493068
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 208 kDa).
One of our customer have mentioned using WB protocol published in J. Biol. Chem. 2010, 285:10362-10369 with lymphoblastoid cell.
Use at 2-5 µg/mg of lysate.
FunctionInvolved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis.
Sequence similaritiesBelongs to the AAA ATPase family.
Post-translational modificationsATR may stimulate the RAD9A dissociation.
Detection of Atad5 by Western Blot of Immunprecipitate.
ab72111 at 1µg/ml staining Atad5 in HeLa whole cell lysate immunoprecipitated using ab72111 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-Atad5 antibody (ab72111)
This product has been referenced in:
Shiomi Y et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol32:2279-88 (2012).
Read more (PubMed: 22493068) »
Rahman S et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol31:2641-52 (2011).
Read more (PubMed: 21555454) »