The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa.
Use at an assay dependent dilution.
RelevanceSignaling through G-proteins is highly conserved in eukaryotes. G-proteins are responsible for transferring extracellular signals from cell-surface receptors. Upon ligand binding to G-protein coupled receptor (GPCR) there is a GDP/GTP exchange on the a-subunit of G proteins (Ga), causing a dissociation of Ga- from Gbg dimer. The Arabidopsis genome contains genes encoding one G-protein a-subunit (GPA1), one b-subunit (AGB1), and two g-subunits (AGG1 and AGG2). Studies on the null alleles of GPA1 and AGB1 showed that plants use heterotrimeric G-protein signaling in several growth and developmental processes. Abscisic acid (ABA) regulates many physiological processes like seed germination, early seeding development, stomatal guard cell functions and acclimation to adverse environmental conditions. Knocking out GPA1 and/or AGB1 in Arabidopsis plants negatively regulates abscisic acid signaling in seed germination and in early seedling development. The lack of AGB1 causes a greater ABA hypersensitivity than the lack of GPA1, suggesting that AGB1 is the predominant regulator of ABA signaling.