Overview

  • Product name
  • Description
    Rabbit polyclonal to Ataxin 1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide, corresponding to amino acids 741-791 of Human Ataxin 1

  • Positive control
    • NIH3T3 cells and Human lung carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab110833 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function.
  • Tissue specificity
    Widely expressed throughout the body.
  • Involvement in disease
    Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities
    Belongs to the ATXN1 family.
    Contains 1 AXH domain.
  • Domain
    The AXH domain is required for interaction with CIC.
  • Post-translational
    modifications
    Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
    Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.
  • Cellular localization
    Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • alternative ataxin1 antibody
    • Ataxin-1 antibody
    • ATX1 antibody
    • ATX1_HUMAN antibody
    • Atxn1 antibody
    • D6S504E antibody
    • OTTHUMP00000016065 antibody
    • SCA1 antibody
    • Spinocerebellar ataxia type 1 protein antibody
    see all

Anti-Ataxin 1 antibody images

  • ab110833 at 1/50 staining Ataxin 1 in paraffin-embedded Human lung carcinoma tissue, left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.
  • ab110833 at 1/100 staining Ataxin in NIH3T3 cells by immunofluorescence, left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.

References for Anti-Ataxin 1 antibody (ab110833)

ab110833 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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