Anti-Ataxin 1 (phospho S776) antibody (ab63376)
Key features and details
- Rabbit polyclonal to Ataxin 1 (phospho S776)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ataxin 1 (phospho S776) antibody
See all Ataxin 1 primary antibodies -
Description
Rabbit polyclonal to Ataxin 1 (phospho S776) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Ataxin 1 aa 700-800 (phospho S776).
Database link: P54253 -
General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
Target
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Function
Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function. -
Tissue specificity
Widely expressed throughout the body. -
Involvement in disease
Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the ATXN1 family.
Contains 1 AXH domain. -
Domain
The AXH domain is required for interaction with CIC. -
Post-translational
modificationsPhosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. -
Cellular localization
Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6310 Human
- Omim: 601556 Human
- SwissProt: P54253 Human
- Unigene: 434961 Human
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Alternative names
- alternative ataxin1 antibody
- Ataxin-1 antibody
- ATX1 antibody
see all
Images
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All lanes : Anti-Ataxin 1 (phospho S776) antibody (ab63376) at 1/500 dilution
Lane 1 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours)
Lane 2 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab63376 has been referenced in 2 publications.
- Brown AS et al. MTSS1/Src family kinase dysregulation underlies multiple inherited ataxias. Proc Natl Acad Sci U S A 115:E12407-E12416 (2018). PubMed: 30530649
- Hearst SM et al. Dopamine D2 receptor signaling modulates mutant ataxin-1 S776 phosphorylation and aggregation. J Neurochem 114:706-16 (2010). ICC/IF ; Human . PubMed: 20477910