Overview

  • Product name
    Anti-Ataxin 1 (phospho S776) antibody
    See all Ataxin 1 primary antibodies
  • Description
    Rabbit polyclonal to Ataxin 1 (phospho S776)
  • Specificity
    Detects Human Ataxin 1 only when phosphorylated at serine 776 (mouse equivalent: serine 752).
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphpeptide derived from human Ataxin 1 around the phosphorylation site of serine 776 (RWSPAP)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
ELISA 1/10000.

Target

  • Function
    Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function.
  • Tissue specificity
    Widely expressed throughout the body.
  • Involvement in disease
    Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities
    Belongs to the ATXN1 family.
    Contains 1 AXH domain.
  • Domain
    The AXH domain is required for interaction with CIC.
  • Post-translational
    modifications
    Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
    Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.
  • Cellular localization
    Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • alternative ataxin1 antibody
    • Ataxin-1 antibody
    • ATX1 antibody
    • ATX1_HUMAN antibody
    • Atxn1 antibody
    • D6S504E antibody
    • OTTHUMP00000016065 antibody
    • SCA1 antibody
    • Spinocerebellar ataxia type 1 protein antibody
    see all

Images

  • All lanes : Anti-Ataxin 1 (phospho S776) antibody (ab63376) at 1/500 dilution

    Lane 1 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours)
    Lane 2 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 87 kDa
    Observed band size : 87 kDa
  • ab63376 staining Ataxin 1 (phospho S776) in human HEK cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.01% Triton X-100 and then blocked using 30% goat serum for 30 minutes at 37°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 37°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 594 (red) used at a 1/400 dilution.

    See Abreview

References

This product has been referenced in:
  • Hearst SM  et al. Dopamine D2 receptor signaling modulates mutant ataxin-1 S776 phosphorylation and aggregation. J Neurochem 114:706-16 (2010). ICC/IF ; Human . Read more (PubMed: 20477910) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK)
Specification
HEK
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.01% Triton
Blocking step
Goat Serum as blocking agent for 30 minute(s) · Concentration: 30% · Temperature: 37°C
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Submitted Sep 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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