Anti-Ataxin 2-like antibody (ab99304)

Overview

  • Product name
    Anti-Ataxin 2-like antibody
    See all Ataxin 2-like primary antibodies
  • Description
    Rabbit polyclonal to Ataxin 2-like
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 150-200 of Human Ataxin 2-like protein (NP_009176.2).

  • Positive control
    • Whole cell lysate from HeLa and 293T cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab99304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Tissue specificity
    Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.
  • Sequence similarities
    Belongs to the ataxin-2 family.
  • Post-translational
    modifications
    Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.
    Arg-361 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2D antibody
    • A2LG antibody
    • A2LP antibody
    • A2RP antibody
    • Ataxin 2 domain protein antibody
    • Ataxin 2 like antibody
    • Ataxin 2 like protein antibody
    • Ataxin 2 related protein antibody
    • Ataxin-2 domain protein antibody
    • Ataxin-2 like antibody
    • Ataxin-2-like protein antibody
    • Ataxin-2-related protein antibody
    • ATX2L_HUMAN antibody
    • ATXN2L antibody
    • ATXN2L ataxin 2 like antibody
    see all

Images

  • All lanes : Anti-Ataxin 2-like antibody (ab99304) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa


    Exposure time : 30 seconds
  • ab99304, at 0.1 µg/ml, staining Ataxin 2-like in HeLa whole cell lysate immunoprecipitated using ab99304 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab99304 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for your interest our AbTrial program.

While ab99304 has not been tested for use on mouse samples, the immunogen is 100% homologous with the mouse sequence. With our AbTrial program, you can now use our products in an unte...

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