Ataxin 7 is a protein of unknown function. It may be the human orthologue of the yeast SAGA SGF73 subunit and a subunit of the human TFTC-like transcriptional complexes.
Spinocerebellar ataxia 7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded trinucleotide repeat in the gene encoding ataxin 7.
Ataxin 7 is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.