Overview

  • Product nameAnti-Ataxin 7 antibody
    See all Ataxin 7 primary antibodies
  • Description
    Rabbit polyclonal to Ataxin 7
  • Tested applicationsSuitable for: ICC/IF, ICC, IP, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human, Drosophila melanogaster
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Ataxin 7 aa 1-17.
    Sequence:

    MSERAADDVRGEPRRAA


    (Peptide available as ab39761)

  • Positive control
    • Whole cell lysate from COS-1 cells transfected with Ataxin 7.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab11434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ICC 1/500.
IP Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa.Can be blocked with Human Ataxin 7 peptide (ab39761).

Target

  • RelevanceAtaxin 7 is a protein of unknown function. It may be the human orthologue of the yeast SAGA SGF73 subunit and a subunit of the human TFTC-like transcriptional complexes. Spinocerebellar ataxia 7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded trinucleotide repeat in the gene encoding ataxin 7. Ataxin 7 is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
  • Cellular localizationCytoplasmic (isoform b) and Nuclear (isoform a)
  • Database links
  • Alternative names
    • ADCAII antibody
    • ATXN 7 antibody
    • OPCA III antibody
    • OPCA3 antibody
    • SCA 7 antibody
    • SCA7 antibody
    • Spinocerebellar Ataxia 7 antibody
    • Spinocerebellar ataxia type 7 protein antibody
    see all

Anti-Ataxin 7 antibody images

  • Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.
  • ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Ataxin 7 antibody (ab11434)

ab11434 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"