Overview

  • Product nameAnti-ATBF1 antibody
    See all ATBF1 primary antibodies
  • Description
    Mouse monoclonal to ATBF1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    Recombinant fragment: EDFESPSMSS VNLNFDQTKL DNDDCSSVNT AITDTTTGDE GNADNDSATG IATETKSSS APNEGLTKAA MMAMSEYEDR LSSGLVSPAP SFYSKEYDNE G, corresponding to internal sequence amino acids 2811-2911 of Human ATBF1 with a 26 kDa tag. (NP_008816).

Properties

Applications

Our Abpromise guarantee covers the use of ab54853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged ATBF1 is approximately 0.3ng/ml if used as a capture antibody.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • RelevanceATBF1 is a transcription factor that negatively regulates alpha fetoprotein and MYB, transactivates CDKN1A, and may be a tumor suppressor. Loss of ATBF1 is one mechanism that defines the absence of growth control in prostate cancer. Presence of the isoform ABTF1-A is correlated with better prognosis in breast cancer and may also serve as a marker of endocrine responsiveness.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Alpha fetoprotein enhancer binding protein antibody
    • AT binding transcription factor 1 antibody
    • AT motif binding factor antibody
    • ATBT antibody
    • ZFHX3 antibody
    see all

Anti-ATBF1 antibody images

  • Anti-ATBF1 antibody (ab54853) at 1/500 dilution + Recombinant tagged fragment of human ATBF1 at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-ATBF1 antibody (ab54853)

ab54853 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"