The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
An additional band at 50 kDa may be observed representing AtEB1b degradation products. Staining of AtEB1b in immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMicrotubules (MT) play a central role in many cellular functions including: intracellular transport, maintenance of cell shape and polarity, and chromosome segregation. In most cells, the minus-ends of microtubules are anchored at the centrosome, whereas plus-ends are more dynamic and responsible for contacting targets such as cell cortex and chromosomes. Several factors regulate MT dynamics in cells. EB1 (End-Binding protein 1) proteins belong to a distinct family of accessory proteins, termed "plus-end tracking proteins" or "+TIPs", that bind to the
plus-ends of microtubules and are thought to play an important role in regulating dynamics and organization of microtubules. These microtubule-associated proteins (MAPs) are among the most conserved components of the MT cytoskeleton. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.