Overview

  • Product name
  • Description
    Rabbit polyclonal to ATF-4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse ATF-4 aa 1-50 (N terminal).
    Sequence:

    MALFTKSSSSVAVTDKDTFELSTFLESSKAPQHDRDELPEQRSVGGGLDD


    Database link: P18848

  • Positive control
    • NIH3T3 cell lysate; Human brain cerebellum tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.125 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • Anti-ATF-4 antibody (ab105383) at 0.125 µg/ml + NIH3T3 cell lysate at 10 µg

    Predicted band size : 42 kDa
    Gel concentration: 12%
  • ab105383, at 5 µg/ml, staining ATF-4 in paraffin-embedded Human brain cerebellum by Immunohistochemistry.

References

This product has been referenced in:
  • Piret SE  et al. A mouse model for inherited renal fibrosis associated with endoplasmic reticulum stress. Dis Model Mech 10:773-786 (2017). IHC-P ; Mouse . Read more (PubMed: 28325753) »
  • Drivas TG  et al. Basal exon skipping and genetic pleiotropy: A predictive model of disease pathogenesis. Sci Transl Med 7:291ra97 (2015). Read more (PubMed: 26062849) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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