Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human ATF-4 peptide (ab22854).

Target

  • FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Anti-ATF-4 antibody images

References for Anti-ATF-4 antibody (ab1371)

This product has been referenced in:
  • Wang Y  et al. Inhibition of JNK by novel curcumin analog C66 prevents diabetic cardiomyopathy with a preservation of cardiac metallothionein expression. Am J Physiol Endocrinol Metab 306:E1239-47 (2014). Mouse . Read more (PubMed: 24714399) »
  • Gavilán E  et al. GSK-3ß signaling determines autophagy activation in the breast tumor cell line MCF7 and inclusion formation in the non-tumor cell line MCF10A in response to proteasome inhibition. Cell Death Dis 4:e572 (2013). WB ; Human . Read more (PubMed: 23559006) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (vascular smooth muscle cells)
Permeabilization Yes - 0.1 % triton X-100
Specification vascular smooth muscle cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Methanol:Acetone 1:1
Username

Abcam user community

Verified customer

Submitted Dec 29 2015

Reviewing purchases of this product, the lab purchased ab1371 and not ab50546. Furthermore, in the methods section of the paper an anti-goat secondary antibody is mentioned but no goat primary is stated as being used. Therefore we do believe the resear...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande du remplacemen...

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (kidney)
Loading amount 30 µg
Specification kidney
Gel Running Conditions Reduced Denaturing (4-12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3)
Loading amount 50 µg
Specification 3T3
Treatment Cells transfected with plasmid encoding ATF4 with HA tag
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2011

Thank you for getting back to me. I am sorry that I did not provide you with reference to the positive controls for each of these antibodies. The following positive control should be used for ATF4 antibody (ab1371): Raji cell lysate: ab7908 Raj...

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Thank you for your phone call and I apologize for the shortage that you received. I have arranged for a free of charge replacement vial to be sent to you. It is being shipped to your attention on order reference # 99647 and you should receive this t...

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I'm sorry to hear you've had trouble with ab1371. Thank you for taking the time to fill in the self-help section to contact us, the information you have given me is very useful. I think you have taken all the right steps to get the antibody working and...

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Thank you for your enquiry and providing more details of your assay. As we understand from your e-mail, the main problem you are experiencing is that you have a lot of non-specific bands. We have done our Western blot analysis with rabbit anti-g...

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Thank you for your message. As we understand from your e-mail you say that "hamster proteins are usually very similar to rat and mouse proteins". However, we would like to emphasize the fact that we haven't tested this particular antibody on hamster. ...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"