Overview

  • Product name
  • Description
    Rabbit polyclonal to ATF-4
  • Specificity
    Antibody detects endogenous levels of ATF-4 protein around Serine 245.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4.
    Database link: P18848

  • Positive control
    • Breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21933012Fixed with methanol
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function
    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • ab31390 at a 1:50-1:100 dilution staining ATF-4 in human breast carcinoma tissue, using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : Un-treated.

    Right image : Antibody pre-incubated with synthesized peptide.

References

This product has been referenced in:
  • Zhu H  et al. Activating transcription factor 4 promotes esophageal squamous cell carcinoma invasion and metastasis in mice and is associated with poor prognosis in human patients. PLoS One 9:e103882 (2014). IHC-P ; Human . Read more (PubMed: 25078779) »
  • Lenna S  et al. HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells. PLoS One 8:e56123 (2013). IHC-P ; Human . Read more (PubMed: 23441162) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Carl Atkinson

Verified customer

Submitted Apr 06 2013

Thank you for contacting us.

For ab21685 we used human liver carcinoma. I would suggest using the following product as a positive control:
Liver tumor (human): hepatocellular carcinoma tissue slides (ab4877) (http://www.abcam.com/ab487...

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Thank you very much for your kind reply. Indeed, ab50546 has passed our strict quality controls and we have received no complaints about this product, therefore, we assume that the individual vial you had received was faulty which may happen occasi...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this ATF4 antibody. The details you have kindly provided will provide us with vital informati...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our ATF4 antibody ab50546. I appreciate the time you have spent in the laboratory and understand your concerns. It is regret...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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