Overview

  • Product name
    Anti-ATF-4 antibody - C-terminal
    See all ATF-4 primary antibodies
  • Description
    Goat polyclonal to ATF-4 - C-terminal
  • Specificity
    This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4 aa 339-351 (C terminal) (Cysteine residue).
    Sequence:

    C-EEVRKARGKKRVP


    Database link: P18848
    (Peptide available as ab22854)

  • Positive control
    • WB: K562 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human ATF-4 peptide (ab22854).

Target

  • Function
    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • Anti-ATF-4 antibody - C-terminal (ab1371) at 0.3 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Xia X  et al. Arginine Supplementation Recovered the IFN-?-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2a Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells. Mol Cells 39:410-7 (2016). WB . Read more (PubMed: 27025389) »
  • Kuwabara WM  et al. NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. PLoS One 10:e0116410 (2015). WB . Read more (PubMed: 25668518) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (vascular smooth muscle cells)
Permeabilization
Yes - 0.1 % triton X-100
Specification
vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Methanol:Acetone 1:1
Username

Abcam user community

Verified customer

Submitted Dec 29 2015

Reviewing purchases of this product, the lab purchased ab1371 and not ab50546. Furthermore, in the methods section of the paper an anti-goat secondary antibody is mentioned but no goat primary is stated as being used. Therefore we do believe the resear...

Read More

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande du remplacemen...

Read More
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
30 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing (4-12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3)
Loading amount
50 µg
Specification
3T3
Treatment
Cells transfected with plasmid encoding ATF4 with HA tag
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2011

Thank you for getting back to me. I am sorry that I did not provide you with reference to the positive controls for each of these antibodies. The following positive control should be used for ATF4 antibody (ab1371): Raji cell lysate: ab7908 Raj...

Read More

Thank you for your phone call and I apologize for the shortage that you received. I have arranged for a free of charge replacement vial to be sent to you. It is being shipped to your attention on order reference # 99647 and you should receive this t...

Read More

I'm sorry to hear you've had trouble with ab1371. Thank you for taking the time to fill in the self-help section to contact us, the information you have given me is very useful. I think you have taken all the right steps to get the antibody working and...

Read More

Thank you for your enquiry and providing more details of your assay. As we understand from your e-mail, the main problem you are experiencing is that you have a lot of non-specific bands. We have done our Western blot analysis with rabbit anti-g...

Read More

Thank you for your message. As we understand from your e-mail you say that "hamster proteins are usually very similar to rat and mouse proteins". However, we would like to emphasize the fact that we haven't tested this particular antibody on hamster. ...

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up