Human Predicted to work with:
Synthetic peptide corresponding to Human ATF-4 (C terminal). Database link: P18848
HeLa cell lysates.
FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Use at an assay dependent concentration.
FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
Sequence similaritiesBelongs to the bZIP family. Contains 1 bZIP domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
Anti-ATF-4 antibody - ChIP Grade (ab85049) at 1/500 dilution + HeLa cell lysates
Predicted band size : 39 kDa Observed band size : 39 kDa
References for Anti-ATF-4 antibody - ChIP Grade (ab85049)
This product has been referenced in:
Zheng S et al. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol195:217-26 (2015).
Read more (PubMed: 26019273) »
Dahlqvist J et al. siRNA silencing of proteasome maturation protein (POMP) activates the unfolded protein response and constitutes a model for KLICK genodermatosis. PLoS One7:e29471 (2012).
Read more (PubMed: 22235297) »