Overview

  • Product name
    Anti-ATF-4 antibody - ChIP Grade
    See all ATF-4 primary antibodies
  • Description
    Rabbit polyclonal to ATF-4 - ChIP Grade
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4 (C terminal).
    Database link: P18848

  • Positive control
    • WB: HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ChIP Use at an assay dependent concentration.

Target

  • Function
    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • ChIP analysis using ab85049 binding ATF-4 in human macrophages. Samples were incubated with undiluted primary antibody for 12 hours at 4°C. Protein binding was detected using real-time PCR.

    Negative Control: Normal rabbit IgG.

    See Abreview

  • Anti-ATF-4 antibody - ChIP Grade (ab85049) at 1/500 dilution + HeLa cell lysates

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References

This product has been referenced in:
  • Zheng S  et al. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol 195:217-26 (2015). ChIP ; Human . Read more (PubMed: 26019273) »
  • Dahlqvist J  et al. siRNA silencing of proteasome maturation protein (POMP) activates the unfolded protein response and constitutes a model for KLICK genodermatosis. PLoS One 7:e29471 (2012). WB ; Human . Read more (PubMed: 22235297) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 24 h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 22 2014

Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - whole cell (macrophages)
Specification
macrophages
Negative control
normal rabbit IgG
Type
Native ChIP (N-ChIP)
Positive control
old sample
Username

Abcam user community

Verified customer

Submitted Jul 09 2014

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (primary human macrophge)
Specification
primary human macrophge
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 15 2014

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (BMDCs, Whole spleen lysate and CY15 (DC cell line))
Loading amount
75 µg
Specification
BMDCs, Whole spleen lysate and CY15 (DC cell line)
Treatment
Treated or not to thapsigargin
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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