Synthetic peptide corresponding to Human ATF-4.
Database link: P18848
Our Abpromise guarantee covers the use of ab28830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/50 - 1/100. | |
ELISA | 1/10000. | |
ICC/IF | Use at an assay dependent concentration. |
ab28830 staining ATF-4 (phospho S245) in Human MRC5 cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
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