Anti-ATF-4 (phospho S245) antibody (ab28830)

Overview

  • Product nameAnti-ATF-4 (phospho S245) antibody
    See all ATF-4 primary antibodies
  • Description
    Rabbit polyclonal to ATF-4 (phospho S245)
  • Specificityab28830 recognizes Phospho-ATF-4(Ser245).
  • Tested applicationsSuitable for: IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4.
    Database link: P18848

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab28830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Anti-ATF-4 (phospho S245) antibody images

  • ab28830 staining ATF-4 (phospho S245) in Human MRC5 cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830
  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830 treated with synthesised phospho-peptide.

References for Anti-ATF-4 (phospho S245) antibody (ab28830)

This product has been referenced in:
  • Kalamida D  et al. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS One 9:e102408 (2014). WB ; Human . Read more (PubMed: 25010689) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (MRC5)
Specification MRC5
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
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Dr. Dimitra Kalamida

Verified customer

Submitted Dec 12 2013

Thank you for your inquiry. I am happy to confirm that we have a variety of antibodies against targets that are considered related with ER stress: ATF6, bip, Calnexin, IRE1a, PDI, CHOP, Perk, Ero1 Lalpha To find these antibodies please type in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"