- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-ATF-4 (phospho S245) antibody
See all ATF-4 primary antibodies -
DescriptionRabbit polyclonal to ATF-4 (phospho S245)
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Host speciesRabbit
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Specificityab28830 recognizes Phospho-ATF-4(Ser245).
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Tested applicationsSuitable for: IHC-P, ELISA, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human ATF-4.
Database link: P18848 -
Positive control
- Breast carcinoma
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Corresponding non-phospho antibody
Applications
Our Abpromise guarantee covers the use of ab28830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. | |
| ELISA | 1/10000. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
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Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
- Information by UniProt
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Database links
- Entrez Gene: 468 Human
- Omim: 604064 Human
- SwissProt: P18848 Human
- Unigene: 496487 Human
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Alternative names
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 (phospho S245) antibody (ab28830)This image is courtesy of an Abreview submitted by Dimitra Kalamidaab28830 staining ATF-4 (phospho S245) in Human MRC5 cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830 treated with synthesised phospho-peptide.
References
This product has been referenced in:
- El-Khattouti A et al. Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-?B inhibition. J Cell Mol Med 20:266-86 (2016). WB . Read more (PubMed: 26578344) »
- Kalamida D et al. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS One 9:e102408 (2014). WB ; Human . Read more (PubMed: 25010689) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"