The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
Involvement in diseaseDefects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 KID (kinase-inducible) domain.
RNA binding protein activating transcription factor 1 fusion protein antibody
TREB 36 antibody
TREB36 protein antibody
Anti-ATF1 antibody images
Immunoprecipitation - ATF1 antibody (ab110869)
Detection of ATF1 by Western Blot of Immunprecipitate.
anti-ATF1 at 0.4µg/ml staining ATF1 in HeLa whole cell lysate immunoprecipitated using ab110869 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-ATF1 antibody (ab110869)
has not yet been referenced specifically in any publications.
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