Overview

  • Product name
  • Description
    Rabbit polyclonal to ATF1
  • Specificity
    Detects endogenous levels of total ATF1 protein.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human ATF1 around the phosphorylation site of serine 63 (R-P-SP-Y-R)

  • Positive control
    • Human lung carcinoma tissue COLO cell extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
  • Involvement in disease
    Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
  • Sequence similarities
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 1 antibody
    • ATF 1 antibody
    • atf1 antibody
    • ATF1 EWS fusion gene antibody
    • ATF1 FUS fusion gene antibody
    • ATF1_HUMAN antibody
    • cAMP dependent transcription factor 1 antibody
    • cAMP-dependent transcription factor ATF-1 antibody
    • Cyclic AMP dependent transcription factor ATF 1 antibody
    • Cyclic AMP dependent transcription factor ATF1 antibody
    • Cyclic AMP-dependent transcription factor ATF-1 antibody
    • EWS AFT1 antibody
    • FUS ATF 1 antibody
    • FUS/ATF 1 antibody
    • Protein TREB36 antibody
    • RNA binding protein activating transcription factor 1 fusion protein antibody
    • TREB 36 antibody
    • TREB36 antibody
    • TREB36 protein antibody
    see all

Anti-ATF1 antibody images

  • All lanes : Anti-ATF1 antibody (ab78903) at 1/500 dilution

    Lane 1 : COLO cell extract
    Lane 2 : COLO cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab78903, at 1/50 dilution. Right panel was also treated with immunising peptide.

References for Anti-ATF1 antibody (ab78903)

ab78903 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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