Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

Overview

  • Product nameAnti-ATF1 (phospho S63) antibody [EP1591(2)Y]
    See all ATF1 primary antibodies
  • Description
    Rabbit monoclonal [EP1591(2)Y] to ATF1 (phospho S63)
  • Specificityab76085 detects ATF1 when phosphorylated at serine 63
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF1

  • Positive control
    • WB: HeLa cell lysate IHC-P: human breast carcinoma
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 22052915
WB 1/50000 - 1/100000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
    • Involvement in diseaseDefects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
    • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating transcription factor 1 antibody
      • ATF 1 antibody
      • atf1 antibody
      • ATF1 EWS fusion gene antibody
      • ATF1 FUS fusion gene antibody
      • ATF1_HUMAN antibody
      • cAMP dependent transcription factor 1 antibody
      • cAMP-dependent transcription factor ATF-1 antibody
      • Cyclic AMP dependent transcription factor ATF 1 antibody
      • Cyclic AMP dependent transcription factor ATF1 antibody
      • Cyclic AMP-dependent transcription factor ATF-1 antibody
      • EWS AFT1 antibody
      • FUS ATF 1 antibody
      • FUS/ATF 1 antibody
      • Protein TREB36 antibody
      • RNA binding protein activating transcription factor 1 fusion protein antibody
      • TREB 36 antibody
      • TREB36 antibody
      • TREB36 protein antibody
      see all

    Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] images

    • All lanes : Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anit rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Immunohistochemical analysis of ATF1 in paraffin embedded human breast carcinoma tissue, using ab76085 at a 1/100 dilution.

    References for Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

    This product has been referenced in:
    • Lee CJ  et al. Fibroblast and Epidermal Growth Factors Utilize Different Signaling Pathways to Induce Anchorage-independent Cell Transformation in JB6 Cl41 Mouse Skin Epidermal Cells. J Cancer Prev 19:199-208 (2014). Read more (PubMed: 25337589) »
    • Shihan M  et al. Dehydroepiandrosterone sulfate mediates activation of transcription factors CREB and ATF-1 via a Ga11-coupled receptor in the spermatogenic cell line GC-2. Biochim Biophys Acta 1833:3064-3075 (2013). ICC/IF ; Human . Read more (PubMed: 23988737) »

    See all 3 Publications for this product

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    The concentration of ab76085 batch:GR68333 is 0.2150 mg/ml.

    This a tissue culture supernatant and not affinity purified antibody. As the datasheet indicates ab76085 is a rabbit monoclonal antibody which is...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"