• Product nameAnti-ATF2 antibody
    See all ATF2 primary antibodies
  • Description
    Rabbit polyclonal to ATF2
  • SpecificityThis antibody detects endogenous levels of ATF2 protein around Threonine 73 or 55.
  • Tested applicationsSuitable for: WB, ELISA, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide around the phosphorylation site of Threonine 73 or 55 (Human)

  • Positive control
    • IHC-P: Breast Carcinoma


Associated products


Our Abpromise guarantee covers the use of ab31483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA 1/20000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • Tissue specificityAbundant expression seen in the brain.
  • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 antibody images

  • Ab31483, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma tissue byImmunohistochemistry.
    Left image: Using ab31483.
    Right image: The same antibody preincubated with synthesized peptide.
  • All lanes : Anti-ATF2 antibody (ab31483) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells (5-30ug) treated with ab31483.
    Lane 2 : Extracts from LOVO cells (5-30ug) treated using the same antibody preincubated with synthesized peptide.

    goat-anti-rabbit IgG-AP-conjugate.

    Predicted band size : 54 kDa

References for Anti-ATF2 antibody (ab31483)

ab31483 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (T cells)
Loading amount 25 µg
Specification T cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5

Dr. Karppiah Muthumani

Verified customer

Submitted Feb 20 2007