• Product nameAnti-ATF2 antibody
    See all ATF2 primary antibodies
  • Description
    Rabbit polyclonal to ATF2
  • Specificityab53053 detects endogenous levels of ATF2 protein around Threonine 51 or 53.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic non-phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 51 or 53 (Q-T-P-T-P).

  • Positive control
    • Human breast carcinoma tissue and LOVO cell extracts.


Associated products


Our Abpromise guarantee covers the use of ab53053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/20000.


  • FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • Tissue specificityAbundant expression seen in the brain.
  • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 antibody images

  • All lanes : Anti-ATF2 antibody (ab53053) at 1/500 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab53053 at 1/50 dilution staining AFT2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References for Anti-ATF2 antibody (ab53053)

ab53053 has not yet been referenced specifically in any publications.

Product Wall

Sorry for the delay in getting back to you. I now have confirmation of the IHC protocol used with Anti-ATF2 antibody (ab53053) to obtain the image presented on the datasheet: Deparaffinization: 1. Incubate slide at 60°C for 60 min 2. Deparaffinize ...

Read More