The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
1/250 - 1/500.
Is unsuitable for Flow Cyt.
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Abundant expression seen in the brain.
Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger.
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Immunohistochemical analysis of paraffin-embedded human carcinoma using ab32061 at a dilution of 1/50
This product has been referenced in:
Li Q et al. ATF2 translation is induced under chemotherapeutic drug-mediated cellular stress via an IRES-dependent mechanism in human hepatic cancer Bel7402 cells. Oncol Lett12:4795-4802 (2016).
Read more (PubMed: 28105187) »