Overview

  • Product name
    Anti-ATF2 antibody [E243]
    See all ATF2 primary antibodies
  • Description
    Rabbit monoclonal [E243] to ATF2
  • Specificity
    This antibody recognises ATF2, but does not cross react with other ATF family members.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in C terminus of human ATF2.

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Breast carcinoma tissue. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-ATF2 antibody (HRP) [E243] (ab200174)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/250 - 1/500.
ICC 1/250 - 1/500.
IP 1/60.
Flow Cyt 1/2000.

Target

  • Function
    Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • Tissue specificity
    Abundant expression seen in the brain.
  • Sequence similarities
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    modifications
    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Images

  • Anti-ATF2 antibody [E243] (ab32160) at 1/10000 dilution + HeLa cell lysate

    Predicted band size : 54 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.
  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATF2 (red) with ab32160 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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