Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 54 kDa).
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger.
Post-translational modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
These images show human breast carcinoma tissue stained with ab53023 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
Western blot - ATF2 (phospho T51 + T53) antibody (ab53023)
Anti-ATF2 (phospho T51 + T53) antibody (ab53023) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution