Anti-ATF2 (phospho T51 + T53) antibody (ab53023)


  • Product nameAnti-ATF2 (phospho T51 + T53) antibody
    See all ATF2 primary antibodies
  • Description
    Rabbit polyclonal to ATF2 (phospho T51 + T53)
  • SpecificityThis antibody detects endogenous levels of ATF2 only when phosphorylated at threonine 51 or threonine 53.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from human ATF2 around the phosphorylation site of threonine 51 or threonine 53 (Q-TP-P-TP-P)

  • Positive control
    • Breast carcinoma tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab53023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 54 kDa).


  • FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • Tissue specificityAbundant expression seen in the brain.
  • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • Post-translational
    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 (phospho T51 + T53) antibody images

  • These images show human breast carcinoma tissue stained with ab53023 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
  • Anti-ATF2 (phospho T51 + T53) antibody (ab53023) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 54 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ATF2 (phospho T51 + T53) antibody (ab53023)

ab53023 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab53023.
Please use the links above to contact us or submit feedback about this product.