Anti-ATF2 (phospho T69 + T71) antibody [ATF-22P] (HRP) (ab49625)

Overview

  • Product name
    Anti-ATF2 (phospho T69 + T71) antibody [ATF-22P] (HRP)
    See all ATF2 primary antibodies
  • Description
    Mouse monoclonal [ATF-22P] to ATF2 (phospho T69 + T71) (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, ICC, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Leaf frog
  • Immunogen

    Synthetic peptide:

    ADQTPTPTRFLKNC

    conjugated to KLH, corresponding to N terminal amino acids 66-79 of Human ATF2

  • Positive control
    • Whole extract of cultured human acute T cell leukemia Jurkat cells activated with anisomycin.
  • General notes
    Molar ratio Ab/E: 0.6-1.2 Enzyme activity: 150-500 U/ml

Properties

Applications

Our Abpromise guarantee covers the use of ab49625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 52 kDa. Actual band: triplet at 60-70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
    • Tissue specificity
      Abundant expression seen in the brain.
    • Sequence similarities
      Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • Post-translational
      modifications
      Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating transcription factor 2 antibody
      • Activating transcription factor 2 splice variant ATF2 var2 antibody
      • ATF 2 antibody
      • Atf-2 antibody
      • Atf2 antibody
      • ATF2 protein antibody
      • ATF2_HUMAN antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP response element binding protein CRE BP1 antibody
      • cAMP response element-binding protein CRE-BP1 antibody
      • cAMP responsive element binding protein 2, formerly antibody
      • cAMP-dependent transcription factor ATF-2 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CRE BP1 antibody
      • CRE-BP antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • CREBP1 antibody
      • Cyclic AMP dependent transcription factor ATF 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-2 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • D130078H02Rik antibody
      • D18875 antibody
      • HB 16 antibody
      • HB16 antibody
      • Histone acetyltransferase ATF2 antibody
      • MGC105211 antibody
      • MGC105222 antibody
      • MGC111558 antibody
      • MGC142504 antibody
      • mXBP antibody
      • MXBP protein antibody
      • Tg(Gzma-Klra1)7Wum antibody
      • TREB 7 antibody
      • TREB7 antibody
      see all

    References for Anti-ATF2 (phospho T69 + T71) antibody [ATF-22P] (HRP) (ab49625)

    ab49625 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab49625.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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