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Synthetic peptide corresponding to Human ATF2 aa 50-150.
Database link: P15336
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab32019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).|
Immunocytochemistry of HeLa (Human epithelial cell line from cervix adenocarcinoma), prepared in FBS free medium overnight labeling ATF2 at 0.9 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/500 . Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counter stain at 2.5 μg/ml. DAPI was used for nuclear counter stain. Confocal image showing the expression was increased on HeLa cells, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30min.
Blocking and diluting buffer used was 5% NFDM/TBST
Blocking and diluting buffer used was 2% BSA/TBST.
Blocking and diluting buffer used was 2% BSA/TBST .
The molecular weight is 70kD, consistent with the literature (PMID: 24223142).
Lane 1 (input): HeLa (human cervix adenocarcinoma) treated with 250ng/ml anisomycin for 30min whole cell lysate 10μg.
Lane 2 (+): HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32019 in HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate.
Ab32019 Immunoprecipitating ATF2 in Human Hela whole cell lysate. For western blotting ab32019 (1:1000) was used to confirm successful immunoprecipitation. Blocking and diluting buffer used was 5% NFDM/TBST.
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