Overview

  • Product name
  • Description
    Rabbit polyclonal to ATF3
  • Specificity
    Detects endogenous levels of total ATF3 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from internal region of human ATF3.

  • Positive control
    • RAW264.7 cell extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/5000.

Target

  • Function
    This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
  • Sequence similarities
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 3 antibody
    • ATF3 antibody
    • ATF3_HUMAN antibody
    • ATF3deltaZip2 antibody
    • ATF3deltaZip2c antibody
    • ATF3deltaZip3 antibody
    • cAMP dependent transcription factor ATF3 antibody
    • cAMP-dependent transcription factor ATF-3 antibody
    • Cyclic AMP dependent transcription factor ATF3 antibody
    • Cyclic AMP-dependent transcription factor ATF-3 antibody
    see all

Images

  • All lanes : Anti-ATF3 antibody (ab70005) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Spassov S  et al. Genetic targets of hydrogen sulfide in ventilator-induced lung injury--a microarray study. PLoS One 9:e102401 (2014). WB ; Mouse . Read more (PubMed: 25025333) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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