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Synthetic peptide corresponding to Human ATF6 (N terminal). 17 a.a. immunogen somewhere between a.a. 25-75
Our Abpromise guarantee covers the use of ab37149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Only Peptide ELISA has been tested.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).|
ab37149 staining human Hella cells by ICC/IF. Cells were PFA fixed and permeabilized for 10 minutes in 0.1% Triton X-100 prior to blocking in 3% BSA for 15 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. The secondary antibody used was Alexa Fluor®568 goat anti-rabbit (ab175471) diluted at 1/1000.
A20 (mouse reticulum sarcoma cell line) cells stained for ATF6 using ab37149 at 10 µg/ml in ICC.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ATF6 (green) using ab37149 at 1/100 dilution in ICC/IF, followed by Goat anti-rabbit Alexa Fluor® 488.