Overview

  • Product name
    Anti-ATF6 antibody - Carboxyterminal end
    See all ATF6 primary antibodies
  • Description
    Rabbit polyclonal to ATF6 - Carboxyterminal end
  • Specificity
    ab62576 only reacts with only ATF6 alpha.
  • Tested applications
    Suitable for: ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human ATF6

  • Positive control
    • ICC/IF: MCF7 cells. WB: EL4 cell lysate. IHC-P: Human breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

Target

  • Function
    Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Domain
    The basic domain functions as a nuclear localization signal.
    The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
  • Post-translational
    modifications
    During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.
    N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).
    Phosphorylated in vitro by MAPK14/P38MAPK.
  • Cellular localization
    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 6 alpha antibody
    • Activating transcription factor 6 antibody
    • ATF 6 antibody
    • ATF6 alpha antibody
    • ATF6 antibody
    • ATF6-alpha antibody
    • ATF6A antibody
    • ATF6A_HUMAN antibody
    • cAMP dependent transcription factor ATF 6 alpha antibody
    • cAMP-dependent transcription factor ATF-6 alpha antibody
    • Cyclic AMP dependent transcription factor ATF 6 alpha antibody
    • DKFZp686P2194 antibody
    • ESTM49 antibody
    • FLJ21663 antibody
    • Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody
    • Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
    see all

Images

  • Paraffin-embedded human breast tissue stained for ATF6 using ab62576 at 5 µg/ml in immunohistochemical analysis.



  • Predicted band size : 75 kDa

    Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/mL.

  • ab62576 at 10µg/ml staining AFT6 in MCF7 cells by Immunocytochemistry.
  • Lane 1 : Anti-ATF6 antibody - Carboxyterminal end (ab62576) at 0.5 µg/ml
    Lane 2 : Anti-ATF6 antibody - Carboxyterminal end (ab62576) at 1 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence of ATF6 in MCF7 cells using ab62576 at 10 ug/ml.

References

This product has been referenced in:
  • Liu J  et al. High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A. Biomed Res Int 2016:4130834 (2016). WB ; Mouse . Read more (PubMed: 27579314) »

See 1 Publication for this product

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