Overview

  • Product name
  • Description
    Rabbit polyclonal to ATF6 beta
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within corresponding to internal sequence amino acids 504-553 (FNRTESLRLA DELSGWVQRH QRGRRKIPQR AQERQKSQPR KKSPPVKAVP) of human ATF6 beta (NP_004372).

  • Positive control
    • Human fetal muscle lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83213 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • Function
    Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN(9)CCAC[GA]-3') when NF-Y is bound to ERSE.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • Domain
    The basic domain functions as a nuclear localization signal.
    The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.
  • Post-translational
    modifications
    N-glycosylated.
    During unfolded protein response an approximative 60 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is probably performed sequentially by site-1 and site-2 proteases.
  • Cellular localization
    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 6 beta antibody
    • ATF 6 beta antibody
    • ATF6-beta antibody
    • ATF6B antibody
    • ATF6B_HUMAN antibody
    • ATF6beta antibody
    • cAMP response element binding protein related protein antibody
    • cAMP response element-binding protein-related protein antibody
    • cAMP responsive element binding protein like 1 antibody
    • cAMP-dependent transcription factor ATF-6 beta antibody
    • cAMP-responsive element-binding protein-like 1 antibody
    • CREB L1 antibody
    • Creb related protein antibody
    • CREB RP antibody
    • Creb-rp antibody
    • CREBL 1 antibody
    • CREBL1 antibody
    • Crebrp antibody
    • Cyclic AMP dependent transcription factor ATF 6 beta antibody
    • Cyclic AMP dependent transcription factor ATF6 beta antibody
    • FLJ10066 antibody
    • G13 antibody
    • G13 protein antibody
    • OTTHUMP00000029432 antibody
    • OTTHUMP00000029433 antibody
    • Processed cyclic AMP-dependent transcription factor ATF-6 beta antibody
    • Protein G13 antibody
    see all

Images

  • Anti-ATF6 beta antibody (ab83213) at 1 µg/ml + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa

References

ab83213 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83213.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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