The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 10 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
Tissue specificityExpressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta.
Sequence similaritiesBelongs to the bZIP family. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger.
Post-translational modificationsOn EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity. Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
Cellular localizationNucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml. Developed using the ECL technique, with an exposure time of 10 seconds.
References for Anti-ATF7 antibody (ab87844)
has not yet been referenced specifically in any publications.
Publishing research using ab87844? Please let us know so that we can cite the reference in this datasheet.
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