Overview

  • Product name
    Anti-ATG101 antibody
  • Description
    Rabbit polyclonal to ATG101
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    16 amino acid peptide near the center of human ATG101 (GenBank accession no. NP_001092143).

  • Positive control
    • Human liver tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Autophagy factor required for autophagosome formation.
  • Sequence similarities
    Belongs to the ATG101 family.
  • Cellular localization
    Cytoplasm. Preautophagosomal structure. Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG101 antibody
    • Atg13-interacting protein antibody
    • ATGA1_HUMAN antibody
    • Autophagy-related protein 101 antibody
    • C12orf44 antibody
    • Chromosome 12 open reading frame 44 antibody
    • FLJ11773 antibody
    • OTTHUMP00000241687 antibody
    • OTTHUMP00000241688 antibody
    • OTTHUMP00000241689 antibody
    see all

Images

  • Immunofluorescence of ATG101 in mouse liver tissue with ab105387 at 20 ?g/mL.
  • Lane 1 : Anti-ATG101 antibody (ab105387) at 1 µg/ml
    Lane 2 : Anti-ATG101 antibody (ab105387) at 2 µg/ml

    Lane 1 : human liver tissue lysate
    Lane 2 : human liver tissue lysate

    Lysates/proteins at 15 µg/ml per lane.


    Predicted band size : 25 kDa
  • Immunohistochemistry of ATG101 in mouse liver with ab105387 antibody at 5 µg/ml

References

ab105387 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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