The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
Use at an assay dependent concentration.
FunctionAutophagy factor required for autophagosome formation.
Sequence similaritiesBelongs to the ATG101 family.
Cellular localizationCytoplasm. Preautophagosomal structure. Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.