Overview

  • Product name
  • Description
    Goat polyclonal to ATG16L1
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ATG16L1 aa 567-581 (internal sequence) (Cysteine residue). NP_110430.5; NP_060444.3.
    Sequence:

    C-KVEKVLSKQHSSSIN


    Database link: Q676U5

  • Positive control
    • WB: Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 68.1 kDa).

Target

  • Function
    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease
    Inflammatory bowel disease 10
  • Sequence similarities
    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization
    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links
  • Form
    There are 4 isoforms produced by alternative splicing.
  • Alternative names
    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Images

  • Anti-ATG16L1 antibody (ab223238) at 0.1 µg/ml + Mouse brain lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 68.1 kDa
    Observed band size: 65 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab223238 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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