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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-ATG16L1 antibody
See all ATG16L1 products (10) ...
Description
Rabbit polyclonal to ATG16L1
Tested applications
Cross reactivity
Reacts with
Human, Chimpanzee, Monkey
Predicted to work with
Mouse, Dog
Immunogen
A portion of amino acids 25-75 of human ATG16L1 (NP_110430).
Positive control
Human skeletal muscle lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
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Western blot - ATG16L1 antibody (ab72086)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab72086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 - 3 µg/ml. Detects bands of approximately 27, 40, 50 and 68 kDa (predicted molecular weight: 68 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays an essential role in autophagy.
Involvement in disease
Genetic variations in ATG16L1 are associated with susceptibility to inflammatory bowel disease type 10 (IBD10) [MIM:611081]. IBD is characterized by a chronic relapsing intestinal inflammation. IBD is subdivided into Crohn disease (CD) and ulcerative colitis phenotypes. IBD10 individuals show the phenotype characteristic to CD. It may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. CD is commonly classified as autoimmune disease.
Sequence similarities
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats.
Cellular localization
Cytoplasm. Preautophagosomal structure membrane. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.
Target information above from: UniProt accessionQ676U5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 4 isoforms produced by alternative splicing.
Alternative names
- FLJ00045 antibody
- FLJ22677 antibody
- A16L1_HUMAN antibody
see all
Database links
- Entrez Gene: 460022 Chimpanzee
- Entrez Gene: 477412 Dog
- Entrez Gene: 55054 Human
- Entrez Gene: 77040 Mouse
- Omim: 610767 Human
- SwissProt: Q676U5 Human
- SwissProt: Q8C0J2 Mouse
- Unigene: 529322 Human
see all
Anti-ATG16L1 antibody images:
Western blot - ATG16L1 antibody (ab72086)
All lanes : Anti-ATG16L1 antibody (ab72086) at 2 µg/ml
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human skeletal muscle lysate plus immunizing peptide
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 27 kDa (possible isoform),50 kDa (possible isoform).
Note: Multiple isoforms of ATG16L1 can be detected in mouse skeletal muscle lysate using ab72086. The approximately 40 kD band seen in this lysate is also seen in prebleed and is not believed to be relevant.
References for Anti-ATG16L1 antibody (ab72086)
ab72086 has not yet been referenced specifically in any publications.
Publishing research using ab72086? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"