Validated using a knockout cell line
Recombinant
RabMAb

Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671)

Overview

  • Product name
    Anti-ATG16L1 antibody [EPR15638] - N-terminal
    See all ATG16L1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15638] to ATG16L1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ATG16L1 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q676U5

  • Positive control
    • HeLa, Raji, Jurkat, PC12 and NIH 3T3 cell lysates; Human prostatic hyperplasia and Mouse colon tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease
    Inflammatory bowel disease 10
  • Sequence similarities
    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization
    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links
  • Form
    There are 4 isoforms produced by alternative splicing.
  • Alternative names
    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ATG16L1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab187671 observed at 68 and 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab187671 was shown to recognize ATG16L1 when ATG16L1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab187671 and ab8245 (loading control to GAPDH) were diluted 1/2000 and 10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATG16L1 with ab187671 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • All lanes : Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa

  • All lanes : Anti-ATG16L1 antibody [EPR15638] - N-terminal (ab187671) at 1/1000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ATG16L1 with ab187671 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

References

This product has been referenced in:
  • Fan X  et al. Foot-and-mouth disease virus infection suppresses autophagy and NF-?B antiviral responses via degradation of ATG5-ATG12 by 3C(pro). Cell Death Dis 8:e2561 (2017). Read more (PubMed: 28102839) »

See 1 Publication for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK)
Gel Running Conditions
Reduced Denaturing
Loading amount
1 µg
Specification
HEK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Ryan Russell

Verified customer

Submitted Sep 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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