Synthetic peptide corresponding to Human ATG16L1 (N terminal). Conjugated to Blue Carrier Protein. Database link: Q676U5
WB: Jurkat and PBMC cell lysates.
ICC/IF: HL-60 cells.
Lyophilised:Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Use under non reducing condition.
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
Involvement in disease
Inflammatory bowel disease 10
Belongs to the WD repeat ATG16 family. Contains 7 WD repeats.
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
Acetone-fixed HL-60 (human promyelocytic leukemia cell line) cells stained for ATG16L1 (red) using ab228498 at 1/100 dilution in ICC/IF, followed by a Goat anti-Rabbit Alexa Fluor® 546 conjugate at 1/100 dilution as secondary antibody.
Nuclear counter stain: Hoechst Dyeblue (blue).
has not yet been referenced specifically in any publications.
Publishing research using ab228498? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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