Anti-ATG16L1 antibody - N-terminal (ab228498)

Overview

  • Product name
    Anti-ATG16L1 antibody - N-terminal
    See all ATG16L1 primary antibodies
  • Description
    Rabbit polyclonal to ATG16L1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATG16L1 (N terminal). Conjugated to Blue Carrier Protein.
    Database link: Q676U5

  • Positive control
    • WB: Jurkat and PBMC cell lysates. ICC/IF: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Use under non reducing condition.
ICC/IF 1/100.

Target

  • Function
    Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
  • Involvement in disease
    Inflammatory bowel disease 10
  • Sequence similarities
    Belongs to the WD repeat ATG16 family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
  • Cellular localization
    Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.
  • Information by UniProt
  • Database links
  • Form
    There are 4 isoforms produced by alternative splicing.
  • Alternative names
    • A16L1_HUMAN antibody
    • APG16 like 1 antibody
    • APG16-like 1 antibody
    • APG16L antibody
    • APG16L beta antibody
    • ATG16 autophagy related 16 like 1 antibody
    • ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody
    • ATG16A antibody
    • ATG16L antibody
    • Atg16l1 antibody
    • Autophagy related protein 16 1 antibody
    • Autophagy-related protein 16-1 antibody
    • FLJ00045 antibody
    • FLJ10035 antibody
    • FLJ10828 antibody
    • FLJ22677 antibody
    • IBD10 antibody
    • OTTHUMP00000164391 antibody
    • OTTHUMP00000164393 antibody
    • OTTHUMP00000165876 antibody
    • OTTHUMP00000165877 antibody
    • WD repeat domain 30 antibody
    • WDR30 antibody
    see all

Images

  • Anti-ATG16L1 antibody - N-terminal (ab228498) at 1/100 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Developed using the ECL technique.

    Performed under non-reducing conditions.
  • Anti-ATG16L1 antibody - N-terminal (ab228498) at 1/100 dilution + PMBC (peripheral blood mononuclear cells) cell lysate

    Developed using the ECL technique.

    Performed under non-reducing conditions.
  • Acetone-fixed HL-60 (human promyelocytic leukemia cell line) cells stained for ATG16L1 (red) using ab228498 at 1/100 dilution in ICC/IF, followed by a Goat anti-Rabbit Alexa Fluor® 546 conjugate at 1/100 dilution as secondary antibody.

    Nuclear counter stain: Hoechst Dyeblue (blue).

References

ab228498 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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